Comparative mapping of human chromosome 3 genes in the pig shows different gene order
نویسنده
چکیده
and Implications A comparative map of human chromosome 3 (HSA3) and pig chromosome 13 (SSC13) was constructed using physically assigned pig sequence tagged sites (STSs). Pig STS representing 11 HSA3 genes were developed and 10 pig STS were regionally mapped using a somatic cell hybrid panel (SCHP) to SSC13 with 80Ð100% concordance. Large-insert probes were obtained by screening a YAC library with primers for each STS. YACs were identified for DRD3, GAP43, PIT1, SI, and SST for fluorescent in situ hybridization (FISH) mapping. Single gene and bicolor FISH with each pairwise combination was used to further define gene order on SSC13. These data confrim chromosome painting results that showed HSA3 probes hybridize to a major portion of pig chromosome 13 and demonstrate extensive gene rearrangements within this conserved synteny group.
منابع مشابه
Comparative mapping of human chromosome 13 genes in the pig shows a similar gene arrangement
Previous comparative mapping between the human and pig genomes suggested complete conservation of human chromosome 13 (HSA13) to pig chromosome 11 (SSC11). The objectives of this study were comparative gene mapping of pig homologs of HSA13 genes and an examination of gene order within this conserved synteny group by physical assignment of each locus. A detailed HSA13 to SSC11 comparison was cho...
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